Laboratory of Prokaryote Genetic Engineering

Laboratory of Prokaryote Genetic Engineering (Head scientist – Dr K. Stankevičius) are focused on various aspects of restriction-modification phenomenon and on the practical application of results. The object of studies is interaction between sequence, structure and functions of restriction-modification constituent parts (restriction endonucleases and DNA methyltransferases). Most of the experiments were carried out worldwide in order to understand DNA recognition mechanisms and to change specificity of restriction endonucleases that are commercially valuable enzymes. As a consequence, the structures of 15 different high quality restriction endonucleases were identified by using X-rays. However, the information received in this way appeared to be insufficient in order to create restriction endonucleases characterized by new specific qualities. The new technology (so-called sampling based on methylation activity) was developed in the Laboratory of Prokaryote Gene Engineering in order to isolate restriction endonucleases of that are characterized by new features. By using this technology, Eco57MI restriction endonucleases with new specific features was isolated for the first time in the world. The studies conducted in the Laboratory of Prokaryote Gene Engineering are directed to the creation of new DNA manipulation instruments.

The main projects of the Laboratory of Prokaryote Genetic Engineering analyze type II bifunctional restriction endonucleases, type IIS restriction endonucleases and the importance of pathogenic microbacterial genes.

Scientists of Laboratory of Prokaryote Genetic Engineering can provide consultation for these questions:

In addition, scientists of the Laboratory of Prokaryote Genetic Engineering can provide members of other institutions with practical assistance in experiments that require the usage of DNA chip scanner GenePixTM4000B from Axon Instruments that is included in the equipment database of this laboratory.